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. 1970 Oct;67(2):951-8.
doi: 10.1073/pnas.67.2.951.

Mechanism of polymerization of the Salmonella O-antigen: utilization of lipid-linked intermediates

S Kanegasaki, A Wright

Mechanism of polymerization of the Salmonella O-antigen: utilization of lipid-linked intermediates

S Kanegasaki et al. Proc Natl Acad Sci U S A. 1970 Oct.

Abstract

Cell envelope fractions from Salmonella can utilize exogenous lipid-linked intermediates for the synthesis of polymeric O-antigen. We describe a method for preparing aqueous suspensions of lipid intermediates and show that freezing and thawing of cell envelope-lipid intermediate mixtures is required for efficient synthesis. The lipid intermediates move freely in the hydrophobic environment of the membrane.

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