Routine chromosome screening in Syrian hamsters (Mesocricetus auratus) using orbital sinus blood

Abstract

Blood from the posterior orbital sinus of Syrian hamsters, obtained under halothane anaesthesia, can be cultured to give large numbers of metaphase chromosome spreads for analysis. The procedure has been used for rapid routine screening of the karyotypes of all offspring in a breeding colony where translocations are present. Results can be obtained within 3 days of collecting the blood sample.