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. 1969 Apr;9(4):510-7.
doi: 10.1016/S0006-3495(69)86401-5.

Ultraviolet inactivation and photoproducts of transforming DNA irradiated at low temperatures

R O Rahn, J K Setlow, J L Hosszu

Ultraviolet inactivation and photoproducts of transforming DNA irradiated at low temperatures

R O Rahn et al. Biophys J. 1969 Apr.

Abstract

Solutions of Haemophilus influenzae transforming DNA were irradiated at temperatures ranging from 25 degrees C to - 196 degrees C. Temperature dependence of the formation of thymine-containing dimers was closely correlated with inactivation of transforming activity; in general, both dimerization and inactivation decreased with decreasing temperature. The fraction of nonphotoreactivable damage increased with increasing dose at low temperatures. The nonphotoreactivable spore-type photoproduct was formed at low temperatures with a maximum at - 100 degrees C, a temperature at which the nonphotoreactivable biological inactivation was also a maximum. Intrastrand cross-linking, like dimer formation, decreased with decreasing irradiation temperature.

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