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. 1971 Jun;20(6):895-900.

The inhibition of formation of migration inhibiting factor (MIF) by various antilymphocyte sera

J Pekárek, J Svejcar, J Johanovský

The inhibition of formation of migration inhibiting factor (MIF) by various antilymphocyte sera

J Pekárek et al. Immunology. 1971 Jun.

Abstract

Antilymphocyte serum was prepared in several species and its effect on the production of migration inhibitory factor (MIF) studied. Antilymphocyte sera in vitro in dilutions as high as 1/3200 inhibited the liberation of MIF by sensitized lymphocytes in the presence of antigen. In contrast antipolymorph and antiglobulin sera were inactive.

Antilymphocyte serum also blocked the formation of MIF when the lymphocytes were preincubated with ALS and then washed before the addition of antigen. The antilymphocyte serum and sera prepared against supernatants containing MIF did not block MIF activity by direct neutralization.

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References

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