doi: 10.3109/00365517909108875.
A rapid polyethylene glycol assay for gastric intrinsic factor
- PMID: 531491
- DOI: 10.3109/00365517909108875
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A rapid polyethylene glycol assay for gastric intrinsic factor
Scand J Clin Lab Invest.
1979 Nov.
Abstract
The use of polyethylene glycol (mol. wt 4000) in a rapid radioassay for intrinsic factor is reported. The assay is based on the observation that polyethylene glycol precipitates the intrinsic factor-vitamin B12 complex in the presence of serum from patients with pernicious anaemia having auto-antibodies against intrinsic factor. The assay was found highly reproducible (CV 1.8%) and well correlated to the classical method using coated charcoal in the separation step.
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