Use of dialyzing culture technique for high yield of Mycoplasma

Abstract

Pollock, Mary E. (University of Minnesota, Minneapolis). Use of dialyzing culture technique for high yield of Mycoplasma. J. Bacteriol. 90:1682-1685. 1965.-The saprophytic pleuropneumonia-like organism, Mycoplasma laidlawii, type A, was cultivated within a dialyzing membrane suspended in a reservoir of uninoculated medium. Generation time was not decreased by this method, but the period of active growth was prolonged, and the final yield was 10-fold that attained by the usual cultural methods. A defined mixture, which alone would not support growth of Mycoplasma, could replace the complex soy peptone-yeast extract medium in the inner membrane vessel of the dialyzing culture flask with no decrease in growth rate or yield.