doi: 10.7164/antibiotics.28.845.
Aminoglycoside 3'-phosphotransferase III, a new phosphotransferase. Resistance mechanism
- PMID: 53228
- DOI: 10.7164/antibiotics.28.845
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Aminoglycoside 3'-phosphotransferase III, a new phosphotransferase. Resistance mechanism
J Antibiot (Tokyo).
1975 Nov.
Free article
Abstract
The aminoglycoside phosphotransferase of Pseudomonas aeruginosa 21-75 was purified by affinity chromatography using dibekacin-Sephadex 4B or lividomycin A-Sepharose 4B followed by DEAE Sephadex A-50 chromatography. It had activities of both the known aminoglycoside 3'-phosphotransferases I and II, and transferred phosphate from ATP to the 3'-hydroxyl group of kanamycin A, ribostamycin and butirosin A and 5-hydroxyl group of lividomycin A. This enzyme was designated aminoglycoside 3'-phosphotransferase III. It showed strong substrate inhibition by kanamycin A and ribostamycin when their concentration exceeded 6 muM. Purification and characterization of this enzyme are reported.
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