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. 1966 May;91(5):1763-6.
doi: 10.1128/jb.91.5.1763-1766.1966.

Chromosomal location of the structural gene for glycerol kinase in Escherichia coli

N R Cozzarelli, E C Lin

Chromosomal location of the structural gene for glycerol kinase in Escherichia coli

N R Cozzarelli et al. J Bacteriol. 1966 May.

Abstract

Cozzarelli, N. R. (Harvard Medical School, Boston, Mass.), and E. C. C. Lin. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli. J. Bacteriol. 91:1763-1766. 1966.-A glycerol kinase mutant site has been mapped by transduction and sexual conjugation. Three-factor crosses with the two procedures yielded the following gene order: arginine-1-methionine-1-glycerol kinase-isoleucine, valine-16. An additional 13 independent glycerol kinase mutant sites mapped in the same region. Since some of the mutants were able to produce a protein serologically indistinguishable from the wild-type enzyme, it is concluded that the region mapped represents the structural gene for the kinase.

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References

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