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. 1966 Sep 2;153(3740):1119-20.
doi: 10.1126/science.153.3740.1119.

Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis

E Z Ron, R E Kohler, B D Davis

Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis

E Z Ron et al. Science. .

Abstract

Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.

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