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. 1979 Dec;140(3):1112-5.
doi: 10.1128/jb.140.3.1112-1115.1979.

Rapid screening procedure for detection of plasmids in streptococci

D J Leblanc, L N Lee

Rapid screening procedure for detection of plasmids in streptococci

D J Leblanc et al. J Bacteriol. 1979 Dec.

Abstract

An enrichment procedure, yielding plasmid deoxyribonucleic acid preparations normally containing less than 5% chromosomal contamination, has been devised for the isolation of plasmids from virtually all species of streptococci. The procedure is rapid, reproducible, and inexpensive, requiring no radioisotopes or density gradient centrifugation. The procedure can be used for routine screening of several hundred isolates in a short period of time, and plasmids obtained from 10- to 20-ml cultures can readily be visualized in agarose gels.

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References

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    1. J Bacteriol. 1979 Feb;137(2):878-84 - PubMed
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