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. 1979 Oct 1;183(1):175-8.
doi: 10.1042/bj1830175.

The incorporation of tritiated retinyl moiety into the active-site lysine residue of bacteriorhodopsin

E Mullen, M G Gore, M Akhtar

The incorporation of tritiated retinyl moiety into the active-site lysine residue of bacteriorhodopsin

E Mullen et al. Biochem J. .

Abstract

Purple membranes were isolated from Halobacterium halobium bleached and regenerated with all-trans-[15-3H]retinal. The incorporation of label was 1.2 mol of retinal/mol of bacterio-opsin. The [3H]retinyl-bacterio-opsin obtained from regeneration was hydrolysed to give tritiated retinyl-lysine, which, on hydrogenation to N-epsilon-perhydro[3H]retinyl-lysine and reaction with 1-fluoro-2,4-dinitrobenzene, gave bis-(2,4-dinitrophenyl)-N-epsilon-perhydro[3H]retinyl-lysine. This result confirmed that the retinyl moiety of the chromophore is attached to an epsilon-amino group of lysine.

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