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. 1969 Sep;17(3):445-56.

The intracellular localization of two antigens after uptake in vivo by peritoneal macrophages from normal mice

J M Rhodes, I Lind, A Birch-Andersen, H Ravn

The intracellular localization of two antigens after uptake in vivo by peritoneal macrophages from normal mice

J M Rhodes et al. Immunology. 1969 Sep.

Abstract

Normal mouse macrophages, which had ingested ferritin labelled with fluorescein isothiocyanate and human serum albumin labelled with tetramethylrhodamine isothiocyanate in vivo, were fixed in formalin and embedded for electron microscopy. The examination of sections 1–2 μ thick and adjacent ultrathin sections showed that the yellow-green fluorescent droplets (due to ferritin-FITC) seen by fluorescence microscopy were in the same position as the ferritin-containing phagolysosomes as seen by electron microscopy.

Normal mouse macrophages, which had ingested 125I-labelled human serum albumin ([125I]HSA) and unlabelled ferritin, were investigated by electron microscopic autoradiography. Both antigens were found to be situated within the same lysosomes.

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References

    1. J Biophys Biochem Cytol. 1961 Feb;9:409-14 - PubMed
    1. J Exp Med. 1964 Apr 1;119:685-96 - PubMed
    1. J Cell Biol. 1963 Apr;17:19-58 - PubMed
    1. J Exp Med. 1968 Feb 1;127(2):263-76 - PubMed
    1. Immunology. 1965 Oct;9(4):403-5 - PubMed

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