Reductive pentose cycle and formate assimilation in Rhodopseudomonas palustris
- PMID: 5354954
- PMCID: PMC250172
- DOI: 10.1128/jb.100.2.890-894.1969
Reductive pentose cycle and formate assimilation in Rhodopseudomonas palustris
Abstract
Rhodopseudomonas palustris assimilated formate autotrophically as carbon dioxide and hydrogen arising from the activity of the formic hydrogenlyase system. Kinetic analyses of cell suspensions pulse-labeled with (14)C-formate or (14)C-bicarbonate showed similar distributions of incorporated radioactivity. In both cases phosphate esters were the first assimilation products. Ribulose diphosphate carboxylase, phosphoribose isomerase, and phosphoribulokinase, characteristic enzymes of the reductive pentose cycle, were present in extracts of cells grown on formate.
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