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. 1975 Oct;2(3):203-7.
doi: 10.1007/BF00356989.

DNA-synthesis on giant nuclear RNA by AMV DNA polymerase

V M Kavsan, A V Rynditch, O P Samarina, G P Georgiev

DNA-synthesis on giant nuclear RNA by AMV DNA polymerase

V M Kavsan et al. Mol Biol Rep. 1975 Oct.

Abstract

The reverse transcription of pre-mRNA isolated from rat liver or mouse Ehrlich ascites carcinoma cells with the aid of hot phenol fractionation technique is described. Pre-mRNA isolated at 85 degrees C is a more active template than the 65 degree C fraction. The addition of oligo(dT) as a primer strongly stimulated the template activity of the 65 degree C fraction. The size of product corresponds to a sedimentation value of 7 S as measured in alkaline sucrose gradient and is essentially less than the size of template.

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