A comparison of the unlabeled enzyme method with immunofluorescence for the evaluation of human immunologic renal disease

Abstract

The application of the unlabeled enzyme method to immunostaining of human renal biopsy material is described. The specificity and sensitivity of this technic were compared with those of standard immunofluorescent staining procedures. The unlabeled enzyme method, although a lengthier procedure, has proven to be as specific and at least as sensitive as conventional fluorescent staining. The advantages of the procedure are its permanence, its compatibility with conventional staining procedures, and the avoidance of fluorescent microscopy for evaluation.