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. 1970 Jan;116(2):161-9.
doi: 10.1042/bj1160161.

Isolation and spectral characterization of phycobiliproteins

F W Teale, R E Dale

Isolation and spectral characterization of phycobiliproteins

F W Teale et al. Biochem J. 1970 Jan.

Abstract

Several phycobiliproteins were prepared chromatographically pure and their absorption, fluorescence-emission, fluorescence-excitation and fluorescence-excitation polarization spectra determined. Changes in these spectra with ionic strength of the aqueous medium and chromoprotein concentration were interpreted in terms of interchromophore energy transfer and protein subunit equilibria. The complexity of the polarization spectra confirms the presence of different types of chromophore, designated sensitizing (;s') and fluorescing (;f'), in a single protein.

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