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. 1979 Dec 1;164(4):471-8.
doi: 10.1016/s0378-4347(00)81549-2.

Measurement of adriamycin (doxorubicin) and its metabolites in human plasma using reversed-phase high-performance liquid chromatography and fluorescence detection

R N Pierce, P I Jatlow

Measurement of adriamycin (doxorubicin) and its metabolites in human plasma using reversed-phase high-performance liquid chromatography and fluorescence detection

R N Pierce et al. J Chromatogr. .

Abstract

We describe a method for measuring adriamycin and its major metabolite, adriamycinol, in plasma, using reversed-phase high-performance liquid chromatography and fluorescence detection. The lower limit of detection is approximately 1 ng/ml for both compounds; within-day coefficients of variation are 3.6% and 4.4% for adriamycin and adriamycinol, respectively. A slight modification of this procedure also allows measurement of aglycone metabolites.

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