Studies on eosinophil leucocyte migration. I. Eosinophil and neutrophil accumulation following antigen-antibody reactions in guinea-pig skin

Abstract

Eosinophil leucocytes migrated into the site of PCA reactions mediated by those fractions of 7S guinea-pig IgG containing IgG₁; neutrophils were associated with IgG₁ and IgG₂. Maximal eosinophil infiltration is seen at 12 hr and was associated with degranulation.

Intradermal histamine was not eosinophilotactic in guinea-pigs.

Preformed antigen–antibody complexes of IgG₁ and IgG₂ both promoted eosinophil and neutrophil migration in guinea-pig skin but slightly more eosinophils were seen following injections of complexes containing IgG₁. Local eosinophilia and PCA activity were mediated by a relatively heat-stable element since these effects were demonstrable even after prolonged heating of fractions containing IgG₁. Eosinophils were seen following injections of Compound 48/80 and this was accompanied by low mast cell counts; however, there was also some associated tissue destruction.