Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Aug;56(2):180-90.
doi: 10.1085/jgp.56.2.180.

Contribution of the excluded volume of bovine serum albumin for solute molecules to the aprent nonsolvent water

G W Borst-Pauwels, D A Goldstein

Contribution of the excluded volume of bovine serum albumin for solute molecules to the aprent nonsolvent water

G W Borst-Pauwels et al. J Gen Physiol. 1970 Aug.

Abstract

Addition of a macromolecule to a solution will give rise to a large excluded volume for the centers of the solute molecules. This will cause an apparent increase in solute concentration which is of the same order of magnitude as that associated with the nonsolvent volumes reported in the literature. A critical examination of one of the procedures used for the determination of nonsolvent water-the vapor pressure method of Hill-is given, and it is concluded that, with the use of this method, it is impossible to detect any significant nonsolvent water surrounding bovine albumin for either sugars or polyols. Generally, data reported in the literature for the nonsolvent water of proteins or other macromolecules will be too high unless they are corrected for the excluded volume.

PubMed Disclaimer

References

    1. J Am Chem Soc. 1949 Feb;71(2):536-41 - PubMed
    1. Biochem J. 1961 Apr;78:827-33 - PubMed
    1. J Physiol. 1959 Mar 12;145(3):658-66 - PubMed
    1. J Gen Physiol. 1967 May;50(5):1311-25 - PubMed
    1. Int Rev Cytol. 1959;8:387-448 - PubMed