Synthesis of glycoprotein, glycolipid, protein, and lipid in synchronized L5178Y cells
- PMID: 5458998
- PMCID: PMC2107982
- DOI: 10.1083/jcb.45.1.23
Synthesis of glycoprotein, glycolipid, protein, and lipid in synchronized L5178Y cells
Abstract
Synthesis of four macromolecular classes found in membranes-glycoprotein, glycolipid, protein, and lipid-was measured as a function of time of the cell cycle in synchronized L5178Y cells. Incorporation of leucine, choline, fucose, glucosamine, or thymidine into the cells, protein, nucleic acid, or lipid was measured by pulse-labeling for (1/2) hr at (1/2) hr intervals after release from the mitotic block. The amount of protein, lipid, glycoprotein, or glycolipid released or secreted into the medium by the L5178Y cells was also measured as a function of time of the cell cycle. Cellular protein was found to be synthesized throughout the cell cycle, with the highest synthesis occurring in the S period; synthesis was depressed in the M period. Cellular glycoprotein was synthesized at approximately the same times as protein, except that the rates of glycoprotein synthesis in the S period relative to other periods were much greater than for protein. Secreted protein was synthesized throughout the cell cycle without any general pattern, except that secretion was elevated in the late S and G(2) periods. Secreted glycoprotein was similar to secreted protein. Cellular lipid and cellular glycolipid were synthesized almost exclusively in the G(2) and M periods; there was no synthesis in the G(1) and S periods. Release or secretion of glycolipid and lipid also occurred in the G(2) and M periods.
Similar articles
-
Inhibition of protein, glycoprotein, ribonucleic acid and deoxyribonucleic acid synthesis by D-glucosamine and other sugars in mouse leukemic cells L5178Y and selective inhibition in SV-3T3 compared with 3T3 cells.Biochim Biophys Acta. 1971 Jun 17;240(1):74-93. doi: 10.1016/0005-2787(71)90515-6. Biochim Biophys Acta. 1971. PMID: 4329927 No abstract available.
-
Cellular membranes: the biosynthesis of glycoprotein and glycolipid in hela cell membranes.Arch Biochem Biophys. 1969 Mar;130(1):573-83. doi: 10.1016/0003-9861(69)90073-3. Arch Biochem Biophys. 1969. PMID: 5778671 No abstract available.
-
Mitochondrial autonomy: incorporation of monosaccharides into glycoprotein by isolated mitochondria.Science. 1969 Apr 11;164(3876):190-2. doi: 10.1126/science.164.3876.190. Science. 1969. PMID: 5774194
-
Vitamin A and the synthesis of a lipid containing mannose in rat liver.Nutr Rev. 1971 Mar;29(3):67-9. doi: 10.1111/j.1753-4887.1971.tb07244.x. Nutr Rev. 1971. PMID: 4930698 Review. No abstract available.
-
Biosynthesis, transport and secretion of immunoglobulin in plasma cells.Histochem J. 1971 Sep;3(5):389-97. doi: 10.1007/BF01005021. Histochem J. 1971. PMID: 4947688 Review. No abstract available.
Cited by
-
Mitochondrial autonomy. Sialic acid residues on the surface of isolated rat cerebral cortex and liver mitochondria.J Cell Biol. 1972 Oct;55(1):147-60. doi: 10.1083/jcb.55.1.147. J Cell Biol. 1972. PMID: 4653414 Free PMC article.
-
Phospholipid synthesis and degradation during the life-cycle of P815Y mast cells synchronized with excess of thymidine.Biochem J. 1970 Sep;119(3):489-92. doi: 10.1042/bj1190489. Biochem J. 1970. PMID: 5500308 Free PMC article.
-
Targeting Lung Cancer Stem Cells: Research and Clinical Impacts.Front Oncol. 2017 May 5;7:80. doi: 10.3389/fonc.2017.00080. eCollection 2017. Front Oncol. 2017. PMID: 28529925 Free PMC article. Review.
-
De novo fatty acid synthesis at the mitotic exit is required to complete cellular division.Cell Cycle. 2014;13(5):859-68. doi: 10.4161/cc.27767. Epub 2014 Jan 13. Cell Cycle. 2014. PMID: 24418822 Free PMC article.
-
De novo lipogenesis at the mitotic exit is used for nuclear envelope reassembly/expansion. Implications for combined chemotherapy.Cell Cycle. 2019 Jul;18(14):1646-1659. doi: 10.1080/15384101.2019.1629792. Epub 2019 Jun 15. Cell Cycle. 2019. PMID: 31203714 Free PMC article.