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. 1970 Jul;10(7):630-45.
doi: 10.1016/S0006-3495(70)86325-1.

Density invariance of cultured Chinese hamster cells with stage of the mitotic cycle

E C Anderson, D F Petersen, R A Tobey

Density invariance of cultured Chinese hamster cells with stage of the mitotic cycle

E C Anderson et al. Biophys J. 1970 Jul.

Abstract

Isopycnic banding of Chinese hamster line CHO cells in Ficoll gradients shows that a population in balanced, exponential growth is very homogeneous with respect to density, the coefficient of variation of the density distribution spectrum being less than 5% of the mean reduced density (i.e. density minus one). Similar measurements on synchronized cultures indicate that reduced density varies by less than 2% around the life cycle. The mean density of CHO cells in F-10 growth medium is calculated to be 1.051 after correction for osmotic effects of the Ficoll gradient.

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References

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