Effect of transition metals on recovery from plasma of the growth-modulating tripeptide glycylhistidyllysine
- PMID: 546910
- DOI: 10.1016/s0021-9673(00)86403-1
Effect of transition metals on recovery from plasma of the growth-modulating tripeptide glycylhistidyllysine
Abstract
Isolation and purification of growth-modulating peptides from biological sources is often accompanied by excessive losses of bioactive material. During the isolation of a growth-modulating tripeptide glycylhistidyllysine (GHL) from human plasma, copper and iron were found to co-isolate with the peptide. Studies with [3H]GHL demonstrated that these metals interfere at several steps of the procedure for the isolation of GHL from plasma (gel filtration chromatography, high-pressure silica-gel). Removal of these metals with an insoluble chelating resin (Cellex 100) enhanced recovery of [3H]GHL from plasma 8-fold. These results suggest that removal of transition metals may aid in the recovery of peptides which are difficult to isolate from biological sources.
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