Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Jun;118(1):35-9.
doi: 10.1042/bj1180035.

A radiochemical assay for glutamine synthetase, and activity of the enzyme in rat tissues

P Lund

A radiochemical assay for glutamine synthetase, and activity of the enzyme in rat tissues

P Lund. Biochem J. 1970 Jun.

Abstract

1. A radiochemical assay for glutamine synthetase has been developed in which an ATP-regenerating system is incorporated to prevent accumulation of inhibitory amounts of ADP. It is particularly suitable for assay of the enzyme in crude tissue extracts containing high adenosine triphosphatase activity. 2. A survey of the distribution of the enzyme in tissues from normal male rats showed that activity is present in liver, brain cortex, kidney cortex, spleen, testis and retina. 3. The K(m) of the enzyme for l-glutamate is approx. 1.5x10(-2)m.

PubMed Disclaimer

References

    1. Enzymologia. 1957 Jul 31;18(5):327-36 - PubMed
    1. J Biol Chem. 1953 Apr;201(2):661-72 - PubMed
    1. Biochemistry. 1962 Jan;1:153-8 - PubMed
    1. Comp Biochem Physiol. 1969 Oct 15;31(2):205-10 - PubMed
    1. Arch Biochem Biophys. 1957 May;68(1):161-71 - PubMed