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. 1976 Feb;73(2):381-5.
doi: 10.1073/pnas.73.2.381.

Partial purification of a serum factor that causes necrosis of tumors

S Green, A Dobrjansky, E A Carswell, R L Kassel, L J Old, N Fiore, M K Schwartz

Partial purification of a serum factor that causes necrosis of tumors

S Green et al. Proc Natl Acad Sci U S A. 1976 Feb.

Abstract

Tumor necrosis can be induced in transplanted mouse methylcholanthrene-induced sarcoma by a tumor necrosis factor in the serum of mice infected with bacillus Calmette-Guérin and given bacterial endotoxin. Sera from normal mice, endotoxin-treated mice, and mice infected with bacillus Calmette-Guérin do not contain this factor. A 20- to 30-fold purification of the serum factor has been achieved by (NH4)2SO4 fractionation, Sephadex G-100 and G-200 gel filtration, and preparative polyacrylamide electrophoresis. Tumor necrosis factor is not bacterial endotoxin. It migrates with alpha-globulins, is made up of at least four subunits, and has a molecular weight of about 150,000. The active factor is a glycoprotein that contains sialic acid and galactosamine.

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