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. 1970 Dec;120(3):559-66.
doi: 10.1042/bj1200559.

The purification and chemical composition of the lipopolysaccharide of Pseudomonas alcaligenes

B A Key, G W Gray, S G Wilkinson

The purification and chemical composition of the lipopolysaccharide of Pseudomonas alcaligenes

B A Key et al. Biochem J. 1970 Dec.

Abstract

1. A method for obtaining lipopolysaccharide free from glycosaminopeptide from isolated cell walls of Pseudomonas alcaligenes is discussed. 2. About 70-75% of the lipopolysaccharide and 86-90% of the isolated lipid A have been accounted for in terms of identifiable components. 3. Hydrolysates of lipid A contain mainly inorganic phosphate, glucosamine, O-phosphorylglucosamine and fatty acids (dodecanoic acid, dodec-2-enoic acid, 3-hydroxydecanoic acid and 3-hydroxydodecanoic acid), of which the last is the main N-acylating acid of the glucosamine backbone. 4. Material corresponding to the polysaccharide moiety of the lipopolysaccharide is extensively degraded. 5. Solubilization of the lipopolysaccharide by using sodium deoxycholate appreciably affects the chemical composition of the material.

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