Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Dec;120(3):653-60.
doi: 10.1042/bj1200653.

Occurrence and formation of the N epsilon-methyl-lysines in myosin and the myofibrillar proteins

M F Hardy, C I Harris, S V Perry, D Stone

Occurrence and formation of the N epsilon-methyl-lysines in myosin and the myofibrillar proteins

M F Hardy et al. Biochem J. 1970 Dec.

Abstract

1. Adult rabbit skeletal-muscle myosin has been shown to contain 1.0 residue of mono-N(in)-methyl-lysine and 3.3 residues of tri-N(in)-methyl-lysine per molecule of molecular weight 500000. 2. The methyl-lysines appear to be located in the subfragment 1 portion of the myosin molecule. 3. Methyl-lysines are not present in actin, tropomyosin, inhibitory factor and calcium-sensitizing factor. 4. Enzymic methylation of histidine and lysine residues of myosin has been demonstrated in vitro. 5. The methylation of histidine and lysine of the total myofibrillar protein occurs after peptide-bond synthesis. 6. Although methylated lysines and 3-methyl-histidine could not be detected by analysis of hydrolysates, radiochemical evidence is provided for the presence of these residues in the soluble-protein fraction of rabbit skeletal muscle.

PubMed Disclaimer

References

    1. Nature. 1969 Aug 23;223(5208):834-5 - PubMed
    1. Biochemistry. 1967 Feb;6(2):528-40 - PubMed
    1. Biochem J. 1960 Jan;74:94-101 - PubMed
    1. Nature. 1968 Feb 3;217(5127):452-3 - PubMed
    1. Biochem J. 1970 Apr;117(2):44P-45P - PubMed