Autoradiographic demonstration of inhomogeneous distribution of sodium in single oocytes of Bufo bufo

Abstract

1. Autoradiography of frozen cells labelled with (22)Na has been used to locate a sequestered fraction of internal Na in the oocyte which exchanges very slowly or not at all with external Li.2. Relative grain density in nucleus and cytoplasm, measured photometrically, was used as an indication of (22)Na distribution within the oocyte. In test experiments grain density fell to 50% within 19 mum of the edge of the section. Owing to the large diameter of the oocyte (> 600 mum) and its nucleus (> 200 mum), this resolution was adequate to determine cytoplasmic/nuclear (C/N) ratios of grain density.3. In oocytes fully loaded with (22)Na, the mean C/N ratio was 0.92 +/- 0.03 (n = 11). After 5 hr exchange in Li-substituted Na-free Ringer solution, the mean C/N ratio was 2.18 +/- 0.04 (n = 11). After 5 hr exchange in Ringer solution as a control, the mean C/N ratio was 1.39 +/- 0.18 (n = 7). The cytoplasm thus contained a fraction of (22)Na inexchangeable with Li, and more slowly exchangeable with Na than that in the nucleus.4. The non-Li-exchangeable fraction of internal Na thus revealed appeared to be quantitatively similar to that already demonstrated by studies of (22)Na fluxes and of internal Na activity by means of Na-sensitive micro-electrodes.