Post-tetanic hyperpolarization produced by an electrogenic pump in dorsal spinocerebellar tract neurones of the cat

Abstract

1. Hyperpolarization following single and repetitive excitation of dorsal spinocerebellar tract (DSCT) neurones of the cat was studied by intracellular recording.2. Hyperpolarization following an antidromic action potential consisted of an initial, brief phase (undershoot) and a late, prolonged phase. The latter hyperpolarization was independent of the membrane potential, whereas the former was reversed in polarity by hyperpolarizing pulses applied across the DSCT cell membrane.3. DSCT neurones showed a prolonged hyperpolarization after a train of antidromic action potentials. The amplitude and duration of the hyperpolarization were dependent on the number and the frequency of action potentials. A similar hyperpolarization was observed following a train of impulses evoked by depolarizing pulses applied through the intracellular electrode.4. There was no detectable conductance change during the post-tetanic hyperpolarization. The latter showed no reversal potential when the membrane potential was altered.5. The half-decay time of the post-tetanic hyperpolarization was lengthened when the cord temperature was lowered. The temperature coefficient (Q(10)) was 2.4 within the range of 31-40 degrees C.6. The amplitude of the undershoot following each action potential was assumed to provide a criterion for the accumulation of the extracellular K(+). Alterations in the amplitude of undershoots during repetitive excitation suggested that the duration of post-tetanic hyperpolarization depends on the accumulation of extracellular K(+) as well as of intracellular Na(+) associated with a train of impulses.7. It is suggested that post-tetanic hyperpolarization is produced by an electrogenic sodium pump. A possible significance of such a hyperpolarization in impulse coding is discussed.