doi: 10.1016/0165-022x(79)90046-0.
Fluorography of tritium-labelled proteins in immunoelectrophoresis
- PMID: 551976
- DOI: 10.1016/0165-022x(79)90046-0
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Fluorography of tritium-labelled proteins in immunoelectrophoresis
J Biochem Biophys Methods.
1979.
Abstract
A method is described for detecting 3H-labelled proteins in immunoelectrophoretic systems performed on agarose gels. The method is based on the incorporation of a polyacrylamide gel into the agarose gel after the electrophoresis. This mixed gel has the characteristics of a polyacrylamide gel, making it possible to use fluorography as has been described for polyacrylamide gels. The applicability of the fluorography method is demonstrated by analyzing 3H-labelled human serum albumin and 3H-labelled pig intestinal brush border proteins by quantitative immunoelectrophoresis.
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