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. 1979;1(1):59-64.
doi: 10.1016/0165-022x(79)90046-0.

Fluorography of tritium-labelled proteins in immunoelectrophoresis

O Norén, H Sjöström

Fluorography of tritium-labelled proteins in immunoelectrophoresis

O Norén et al. J Biochem Biophys Methods. 1979.

Abstract

A method is described for detecting 3H-labelled proteins in immunoelectrophoretic systems performed on agarose gels. The method is based on the incorporation of a polyacrylamide gel into the agarose gel after the electrophoresis. This mixed gel has the characteristics of a polyacrylamide gel, making it possible to use fluorography as has been described for polyacrylamide gels. The applicability of the fluorography method is demonstrated by analyzing 3H-labelled human serum albumin and 3H-labelled pig intestinal brush border proteins by quantitative immunoelectrophoresis.

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