The interaction of normal lymphocytes and cells from lymphoid cell lines. II. Variations relating to cell line source and lymphocyte donor

Abstract

Irradiated LCL cells from several sources activated the blood lymphocytes of a panel of donors. Individual differences were present but were small. Stimulated blood lymphocytes were more potent activators (after X-irradiation) than small lymphocytes in a one-way mixed lymphocyte reaction, indicating that the state of metabolic activity of the `stimulating' lymphocyte affects the level of activation achieved. However, LCL cells incubated for several days after irradiation had not lost stimulatory capacity. Lymphocytes of pigs, rabbits, rats and mice were much less responsive than human lymphocytes. The response of animal lymphocytes generally varied with the species of origin of the serum used in the cultures.