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. 1976 Jan 28;31(1):9-19.
doi: 10.1007/BF00270394.

Cytochemical study of pseudoisocyanine stained human chromosomes

A M Vagner-Capodano, M H Pinna-Delgrossi, A Stahl

Cytochemical study of pseudoisocyanine stained human chromosomes

A M Vagner-Capodano et al. Hum Genet. .

Abstract

Human meiotic and mitotic chromosomes were studied with N-N' diethyl pseudoisocyanine stain. Following methylation and oxydation, the staining allowed microscopic observation of slides with both monochromatic light and fluorescence. In addition, stained preparations can be permanently conserved. Preceeded by diverse methods of chromosome denaturation or 5-BUDR incorporation, PIC lends itself to a large number of banding techniques. Cytochemical study of stained chromosomes demonstrated a certain PIC affinity for DNA although tests performed do not exclude the possibility of PIC reaction with certain proteins.

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