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. 1976;50(4):427-35.
doi: 10.1159/000231532.

Measurement of dengue virus-specific double-stranded ribonucleic acid in infected primate cell extracts by microquantitative complement-fixation methods

A S Lubiniecki, M Pfancuff, J Mitchell

Measurement of dengue virus-specific double-stranded ribonucleic acid in infected primate cell extracts by microquantitative complement-fixation methods

A S Lubiniecki et al. Int Arch Allergy Appl Immunol. 1976.

Abstract

Microquantitative complement-fixation methods have been applied to the detection and quantitation of double-stranded RNA (dsRNA) in cytoplasmic extracts of uninfected and dengue virus-infected primate cell lines. LLC-MK2 and PGLC-33H cells exhibited increased levels of dsRNA following dengue virus infection. Cytoplasmic extracts of infected and uninfected LLC-MK2 cells subjected to density gradient analysis showed that increased levels of dsRNA were associated with structures believed to be virus-specific. Primate cell dsRNA and dengue dsRNA preferentially reacted with rabbit anti-rIn-rCn, while reovirus dsRNA and rAn-containing synthetic duplexes preferentially reacted with anti-rAn-rUn.

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