[Histochemical demonstration of pancreatic B-cells by means of the colloidal iron (Hale) reaction (author's transl)]
- PMID: 55400
- DOI: 10.1007/BF00507695
[Histochemical demonstration of pancreatic B-cells by means of the colloidal iron (Hale) reaction (author's transl)]
Abstract
The stainability of B-cells in islets of Langerhans by means of colloidal iron reaction has been examined using two standard modifications (Graumann resp. Mowry) of the original Hale-reaction. After previous oxidation of the sections with performic acid a strong and selective staining of B-cells was obtained by the use of a colloidal iron reaction based upon Graumann's method. With Mowry's technique B-cells remained unstained. The demonstration of B-cells using a performic acid-colloidal iron reaction has been compared with methods of known selectivity (Aldehyde Fuchsin, Dichlorpseudoisocyanine). By restaining procedures it could be shown that with the three methods the same type of cell i.e. B-cell is stained.
Similar articles
-
[Histochemical demonstration of pancreatic B-cells by the use of a performic acid-colloidal iron/sulfide-silver method (author's transl)].Histochemistry. 1974;41(2):133-9. doi: 10.1007/BF00499128. Histochemistry. 1974. PMID: 4613721 German. No abstract available.
-
[Detection and analysis of aldehyde fuchsin positive fibers in the connective tissue of the human oviductal mucosa. I. Preliminary investigations and demonstration of the morphological substratum (author's transl)].Acta Histochem. 1980;67(1):54-75. Acta Histochem. 1980. PMID: 6778060 German.
-
Colloidal iron staining in renal epithelial neoplasms, including chromophobe renal cell carcinoma: emphasis on technique and patterns of staining.Am J Surg Pathol. 1998 Apr;22(4):419-24. doi: 10.1097/00000478-199804000-00005. Am J Surg Pathol. 1998. PMID: 9537468
-
[Isolated pancreatic islets of Langerhans and insulin biosynthesis (author's transl)].Tanpakushitsu Kakusan Koso. 1979 Jan 1;24(1):28-36. Tanpakushitsu Kakusan Koso. 1979. PMID: 373017 Review. Japanese. No abstract available.
-
Pancreatic islet cell hormones distribution of cell types in the islet and evidence for the presence of somatostatin and gastrin within the D cell.J Histochem Cytochem. 1976 Jul;24(7):883-97. doi: 10.1177/24.7.60437. J Histochem Cytochem. 1976. PMID: 60437 Review. No abstract available.
Cited by
-
Syngeneic transplantation of rat pancreatic islets into the spleen. Light and electron microscopical findings.Int J Pancreatol. 1988 Jan-Feb;3(1):59-72. doi: 10.1007/BF02788224. Int J Pancreatol. 1988. PMID: 3127496
-
On the specificity of insulin staining by Victoria blue 4R.Histochem J. 1985 May;17(5):515-8. doi: 10.1007/BF01003177. Histochem J. 1985. PMID: 3897146 No abstract available.