Fluorescence of the purine and pyrimidine bases of the nucleic acids in neutral aqueous solution at 300 degrees K
- PMID: 5540307
- DOI: 10.1126/science.171.3972.675
Fluorescence of the purine and pyrimidine bases of the nucleic acids in neutral aqueous solution at 300 degrees K
Abstract
Fluorescence of adenine, guanine, cytosine, and uracil at room temperature in neutral aqueous solution has been detected by means of a digital signal accumulation technique. Corrected emission and excitation spectra are presented and compared with low-temperature data. The quantum yields are, respectively, 2.6 x 10(-4), 3.0 x 10(-4), 0.8 x 10(-4), and 0.5 x 10(-4) when the bases are excited at their low-energy absorption maxima.
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