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. 1971 Jan;8(1):87-90.

Extraction of HL-A transplantation antigen in high molecular weight soluble form

D A Davies, J Colombani, D C Viza, U Hämmerling

Extraction of HL-A transplantation antigen in high molecular weight soluble form

D A Davies et al. Clin Exp Immunol. 1971 Jan.

Abstract

A crude membrane extract was made from a typed human spleen and half of the product was solubilized by proteolytic methods previously described. The remaining half was treated with starch stearate and sodium dodecylsulphate and the soluble product compared with the first for its HL-A specificity and behaviour on Sephadex G-200. The product of the starch stearate method had a molecular weight in excess of 200,000 and might be a membrane subunit.

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References

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