Active uptake of Ca++ and Ca plus,plus-activated Mg++ ATPase in red cell membrane fragments
- PMID: 5543418
- PMCID: PMC2203076
- DOI: 10.1085/jgp.57.2.202
Active uptake of Ca++ and Ca plus,plus-activated Mg++ ATPase in red cell membrane fragments
Abstract
Isolated human red blood cell membrane fragments (RBCMF) were found to take up Ca(++) in the presence of ATP.(1) This ATP-dependent Ca(++) uptake by RBCMF appears to be the manifestation of an active Ca(++) transport mechanism in the red cell membrane reported previously (Schatzmann, 1966; Lee and Shin, 1969). The influences of altering experimental conditions on Ca(++)-stimulated Mg(++) ATPase (Ca(++) ATPase) and Ca(++) uptake of RBCMF were studied. It was found that pretreatment of RBCMF at 50 degrees C abolished both Ca(++) ATPase and Ca(++) uptake. Pretreatment of RBCMF with phospholipases A and C decreased both Ca(++) ATPase and Ca(++) uptake, whereas pretreatment with phospholipase D did not significantly alter either Ca(++) ATPase or Ca(++) uptake. Both Ca(++) ATPase and Ca(++) uptake had ATP specificity, similar optimum pH's, and optimum incubation temperatures. From these results, it was concluded that Ca(++) uptake is intimately linked to Ca(++) ATPase.
Similar articles
-
Phosphorylation of the red blood cell membrane during the active transport of C++.J Gen Physiol. 1976 Feb;67(2):251-61. doi: 10.1085/jgp.67.2.251. J Gen Physiol. 1976. PMID: 130465 Free PMC article.
-
Calcium movements across the membrane of human red cells.J Physiol. 1969 Apr;201(2):369-95. doi: 10.1113/jphysiol.1969.sp008761. J Physiol. 1969. PMID: 4238381 Free PMC article.
-
Active calcium ion uptake by inside-out and right side-out vesicles of red blood cell membranes.J Gen Physiol. 1972 Apr;59(4):462-75. doi: 10.1085/jgp.59.4.462. J Gen Physiol. 1972. PMID: 4260495 Free PMC article.
-
Calcium transport in plasma membrane subfractions of rat liver.Prog Clin Biol Res. 1988;252:231-6. Prog Clin Biol Res. 1988. PMID: 3347618
-
Characterization of Mg-ATP-dependent Ca2+ transport in cat pancreatic microsomes.Am J Physiol. 1983 May;244(5):G480-90. doi: 10.1152/ajpgi.1983.244.5.G480. Am J Physiol. 1983. PMID: 6133452
Cited by
-
Influence of sodium and parathyroid hormone on calcium release from intestinal mucosal cells.J Clin Invest. 1974 Sep;54(3):702-9. doi: 10.1172/JCI107808. J Clin Invest. 1974. PMID: 4852449 Free PMC article.
-
Calcium ion transport by pig erythrocyte membrane vesicles.Biochem J. 1974 Sep;142(3):521-6. doi: 10.1042/bj1420521. Biochem J. 1974. PMID: 4282703 Free PMC article.
-
Calcium ion transport across plasma membranes isolated from rat kidney cortex.Biochem J. 1979 Mar 15;178(3):549-57. doi: 10.1042/bj1780549. Biochem J. 1979. PMID: 454364 Free PMC article.
-
Inhibition of active strontium transport from erythrocyte ghosts by internal calcium: evidence for a specificity controlling site.J Membr Biol. 1979 Jul 31;48(3):265-84. doi: 10.1007/BF01872895. J Membr Biol. 1979. PMID: 490627
-
Dependence on calcium concentration and stoichiometry of the calcium pump in human red cells.J Physiol. 1973 Dec;235(2):551-69. doi: 10.1113/jphysiol.1973.sp010403. J Physiol. 1973. PMID: 4271735 Free PMC article.
