Biophysical properties of the longitudinal smooth muscle of the guinea-pig rectum

Abstract

1. The membrane properties of the longitudinal muscle layer of the guinea-pig rectum were studied in hypertonic solution (twice the normal Krebs by addition of sucrose) by the micro-electrode technique. To produce the electrotonic potential and spike, stimulating partitions were used.2. Hypertonic solution hyperpolarized the membrane and increased the membrane resistance. However, no change in the space constant was observed before and after treatment with hypertonic solution.3. The appearance and amplitude of the spike became regular after treatment with hypertonic solution and appearance of the overshoot was consistent.4. The characteristic constants and the conduction velocity were measured in hypertonic solution.(i) The space constant of the membrane was 0.81 mm, the time constant of the electrotonic potential was 83.7 msec and the time constant of the foot of the propagated spike was 8.8 msec.(ii) The conduction velocity of the excitation measured by insertions of the two micro-electrodes was 4.4 cm/sec.(iii) The chronaxie of the membrane was 71.3 msec.5. The results obtained from the present experiments were discussed in relation to the cable theory, and it was concluded that the passive properties of the membrane of the rectal smooth muscle could be explained by the cable equations.6. The specificities of the electrical properties of rectal smooth muscle were compared with muscle from other regions of the alimentary canal.