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. 1971 Jun 11;172(3988):1134-6.
doi: 10.1126/science.172.3988.1134.

Salt extraction of soluble HL-A antigens

R A Reisfeld, M A Pellegrino, B D Kahan

Salt extraction of soluble HL-A antigens

R A Reisfeld et al. Science. .

Abstract

Extraction of cultured human lymphoid cells with hypertonic salt solutions (3 molar potassium chloride) resulted in high recoveries of membrane-associated histocompatibility (HL-A) antigens in soluble form with potent activity and marked immunologic specificity. The active principle was purified by preparative acrylamide-gel electrophoresis. Application of the hypertonic salt extraction method is now yielding sufficient HL-A antigen to begin the elucidation of the molecular basis of transplantation individuality.

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