The electrophoretic mobility of BP8 ascites tumour cells and allergized lymph-node cells after treatment with inflammatory mediators, ptomaines, polyamines, antisera and neuraminidase or heparin

Abstract

The electrophoretic mobility (EPM) of BP8 cells was reduced by 5-HT 175 μg./10⁶ cells or 7·5 μg. bradykinin, but 100 μg. histamine was without effect. Cadaverine di HCl or putrescine di HCl 1000 μg./10⁶ cells and agmatine sulphate or tyramine HCl (3000 μg.) reduced the EPM, but spermine tetra HCl was effective at 200 μg. However, 5 μg./10⁶ cells of the polyamines—poly-l-lysine, poly-d-lysine, poly-l-arginine reduced the EPM of BP8 cells to zero, and with larger doses the cells moved towards the cathode. With protamine sulphate the reduction of EPM varied with log dose and 150 μg. did not reduce the EPM to zero. Heparin (3 i.u.) always completely reversed the effects of polyamines or protamine. Lysine HCl reduced the effect of poly-l-lysine. Poly-l-lysine (2 μg.) or poly-l-arginine (4 μg.) divided allergized lymph-node cells (LNC) from C57 B1 mice into 2 populations one still moving towards the anode and the other moving towards the cathode. Treatment of BP8 cells or allergized LNC with neuraminidase reduced their EPM and increased their sensitivity to poly-l-lysine in the presence of Ca⁺⁺ but reduced it in the absence of Ca⁺⁺. Heparin reversed the effect of neuraminidase.

The mitogens of Phytohaemagglutinin and Pokeweed did not alter the EPM of BP8 cells or allergized LNC.

Anti-BP8 serum from C57 B1 mice lowered the EPM of BP8 cells, an effect partly reversed by heparin. Anti-C3H serum had less effect and human serum had only a slight effect.