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. 1967 Nov;105(2):679-84.
doi: 10.1042/bj1050679.

Quantitiative nucleic acid changes during phytohaemagglutinin-induced lymphocyte transformation in vitro. Dependence of the response on phytohaemagglutinin-serum rati

D R Forsdyke

Quantitiative nucleic acid changes during phytohaemagglutinin-induced lymphocyte transformation in vitro. Dependence of the response on phytohaemagglutinin-serum rati

D R Forsdyke. Biochem J. 1967 Nov.

Abstract

1. Pig and human blood lymphocytes have been grown in culture without replenishment of medium, and stimulated to transform by phytohaemagglutinin. Quantitative nucleic acid changes during this process have been used as an index of transformation. 2. On the first day, cells attach to glass; then they detach and continue transforming. 3. The degree of transformation is dependent on the phytohaemagglutinin/serum ratio, and is independent of cell concentration within the range 0.5x10(6)-2.0x10(6) cells/ml. 4. At low phytohaemagglutinin/serum ratios there is no response; at high phytohaemagglutinin/serum ratios, inhibition appears after the cells have been cultured for a day.

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References

    1. Nature. 1962 Aug 11;195:582-3 - PubMed
    1. Nature. 1965 Aug 21;207(999):818-21 - PubMed
    1. Acta Haematol. 1966;36(5):344-9 - PubMed
    1. Science. 1964 Dec 25;146(3652):1648-54 - PubMed
    1. Proc Natl Acad Sci U S A. 1964 Nov;52:1151-7 - PubMed