Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1967 Nov;105(2):857-67.
doi: 10.1042/bj1050857.

Effect of thrombin on the radioactive nucleotides of human washed platelets

D M Ireland

Effect of thrombin on the radioactive nucleotides of human washed platelets

D M Ireland. Biochem J. 1967 Nov.

Abstract

Radioactive ATP and ADP were found in platelets after incubation of human platelet-rich plasma with either [8-(14)C]adenosine or [8-(14)C]ADP. Treatment of the labelled and washed platelets with thrombin indicated that, though considerable amounts of ATP and ADP were released to the supernatant, radioactive ATP and ADP remained predominantly in the cellular fraction. Breakdown of radioactive ATP took place to form mainly IMP and hypoxanthine, the latter compound appearing in the supernatant. The results indicate the presence of at least two pools of nucleotide in platelets. Evidence is given that the two pools contain approximately the same amounts of ATP plus ADP, and that the ratio of ATP to ADP in the pool released to the supernatant by the action of thrombin is about 0.7-0.8.

PubMed Disclaimer

References

    1. Blood. 1954 Jun;9(6):602-8 - PubMed
    1. Biochem J. 1952 Jan;50(3):404-7 - PubMed
    1. Scand J Clin Lab Invest. 1965;17(6):537-48 - PubMed
    1. Scand J Clin Lab Invest. 1965;17:239-46 - PubMed
    1. Biochem J. 1966 May;99(2):283-96 - PubMed