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. 1977 Apr;4(2):169-72.
doi: 10.1111/j.1365-2125.1977.tb00690.x.

Radioimmunoassay of vinblastine and vincristine

J D Teale, J M Clough, V Marks

Radioimmunoassay of vinblastine and vincristine

J D Teale et al. Br J Clin Pharmacol. 1977 Apr.

Abstract

1 The cytotoxic agent, vinblastine, was conjugated to albumin, using the Mannich reaction. Rabbits immunized with two conjugates, containing differing amounts of hapten, produce antibodies which bound [3H]-vinblastine. 2 Antisera from one rabbit cross-reacted with both vinblastine and vincristine and were used to develop radioimmunoassays for measuring their concentration in plasma. 3 The antisera showed no cross-reactivity with other alkaloids or cytotoxic drugs and provided assays sensitive to a concentration of 2.1 ng vinblastine or 3.8 ng vincristine/ml of plasma added direct to the assay tubes. 4 This is sufficiently sensitive to permit the measurement of plasma vinblastine levels for up to 24 h after the intravenous administration of 15 mg of the drug.

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