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. 1977 Mar 15;162(3):681-7.
doi: 10.1042/bj1620681.

Studies on the cell-free biosynthesis of beta-lactam antibiotics

P E Bost, A L Demain

Studies on the cell-free biosynthesis of beta-lactam antibiotics

P E Bost et al. Biochem J. .

Abstract

Cell walls of Cephalosporium acremonium mycelia were lysed by enzyme preparations from either Helix pomatia (snail) digestive juice or Cytophaga. The yield of protoplasts depended on the lytic-enzyme preparation and the age of the culture, and it increased after the mycelia were pretreated with dithiothreitol. A cell-free preparation, obtained by osmotic lysis of protoplasts, synthesized labelled penicillin N from L-[14C]valine. Approx. 0.03-0.06% of the amino acid was incorporated into penicillin N. Under conditions of penicillin N synthesis, the broken-protoplast preparation failed to produce significant amounts of cephalosporin C or its precursors, deacetylcephalosporin C and deacetoxycephalosporin C.

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