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. 1977 Jul;82(1):303-6.
doi: 10.1093/oxfordjournals.jbchem.a131685.

Calcium-sensitivity of the microtubule reassembly system. Difference between crude brain extract and purified microtubular proteins

E Nishida, H Sakai
Free article

Calcium-sensitivity of the microtubule reassembly system. Difference between crude brain extract and purified microtubular proteins

E Nishida et al. J Biochem. 1977 Jul.
Free article

Abstract

Microtubule reassembly in crude extracts of porcine brain was inhibited by 10(-5) M Ca2+, wherease 10(-3) M Ca2+ was required for inhibition of the reassembly from purified microtubular proteins. This accounts for the apparent discrepancies between the results reported by other investigators. Furthermore, the Ca-sensitivity of the purified microtubular proteins was nearly completely recovered on addition of a fraction obtained from crude brain extract.

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