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. 1977 Aug;201(2):295-302.
doi: 10.1002/jez.1402010213.

Homozygous mouse embryos produced by microsurgery

Homozygous mouse embryos produced by microsurgery

C L Markert et al. J Exp Zool. 1977 Aug.

Abstract

Homozygous mouse blastocysts have been produced following microsurgical removal of one pronucleus from the fertilized egg, diploidization in cytochalasin B, and culture in vitro. This combination of techniques should greatly shorten the time required to obtain homozygous strains of mice.

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