Selective release of chromosomal proteins during limited DNAase 1 digestion of avian erythrocyte chromatin
- PMID: 562236
- DOI: 10.1016/0092-8674(77)90117-9
Selective release of chromosomal proteins during limited DNAase 1 digestion of avian erythrocyte chromatin
Abstract
Duck erythrocyte chromatin has been treated with DNAase 1 under conditions that are known to digest selectively the structural genes coding for globin mRNAs. This limited digestion releases specific sets of nonhistone chromosomal proteins that are not preferentially released during limited digestion with micrococcal nuclease, which does not selectively attack the globin sequences. Analysis of nucleosome monomer and multimer peaks separated on sucrose gradients after limited digestion with micrococcal nuclease shows that the proteins which are released by DNAase 1 digestion remain associated with the chromatin subunits and can be removed by extraction in 0.5 M NaCl. These proteins are tentatively identified as members of the high mobility group (HMG) proteins (originally described by Goodwin, Sanders and Johns, 1973) in terms of their extractability, electrophoretic characteristics and amino acid composition.
Similar articles
-
Fractionation by micrococcal nuclease digestion of Drosophila embryo chromatin: isolation of a fraction enriched in two major nonhistone proteins.Cell Differ. 1983 Jun;12(6):307-16. doi: 10.1016/0045-6039(83)90009-x. Cell Differ. 1983. PMID: 6223703
-
Selective release of HMG nonhistone proteins during DNase digestion of Tetrahymena chromatin at different stages of the cell cycle.Nucleic Acids Res. 1980 Nov 25;8(22):5275-88. doi: 10.1093/nar/8.22.5275. Nucleic Acids Res. 1980. PMID: 6258140 Free PMC article.
-
A study of the localization of high mobility group proteins in chromatin.Can J Biochem. 1978 Jun;56(6):480-91. doi: 10.1139/o78-075. Can J Biochem. 1978. PMID: 667694
-
Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.Proc Natl Acad Sci U S A. 1979 Feb;76(2):630-4. doi: 10.1073/pnas.76.2.630. Proc Natl Acad Sci U S A. 1979. PMID: 284387 Free PMC article.
-
Fractionation of nucleosomes by salt elution from micrococcal nuclease-digested nuclei.J Cell Biol. 1978 Oct;79(1):97-109. doi: 10.1083/jcb.79.1.97. J Cell Biol. 1978. PMID: 701381 Free PMC article.
Cited by
-
DNA-binding properties of a nonhistone chromosomal protein from lymphocytes.Mol Cell Biochem. 1978 May 31;19(3):147-52. doi: 10.1007/BF00225452. Mol Cell Biochem. 1978. PMID: 566377 No abstract available.
-
DNase I sensitivity of transcriptionally active genes in intact nuclei and isolated chromatin of plants.Proc Natl Acad Sci U S A. 1983 Feb;80(3):815-9. doi: 10.1073/pnas.80.3.815. Proc Natl Acad Sci U S A. 1983. PMID: 6219388 Free PMC article.
-
Loss of chromosomal high mobility group proteins HMG1 and HMG2 when mouse neuroblastoma and Friend erythroleukemia cells become committed to differentiation.Proc Natl Acad Sci U S A. 1981 Oct;78(10):5988-92. doi: 10.1073/pnas.78.10.5988. Proc Natl Acad Sci U S A. 1981. PMID: 6458811 Free PMC article.
-
Differential phosphorylation of nuclear nonhistone high mobility group proteins HMG 14 and HMG 17 during the cell cycle.Proc Natl Acad Sci U S A. 1981 Nov;78(11):6944-8. doi: 10.1073/pnas.78.11.6944. Proc Natl Acad Sci U S A. 1981. PMID: 6458819 Free PMC article.
-
Butyrate suppression of histone deacetylation leads to accumulation of multiacetylated forms of histones H3 and H4 and increased DNase I sensitivity of the associated DNA sequences.Proc Natl Acad Sci U S A. 1978 May;75(5):2239-43. doi: 10.1073/pnas.75.5.2239. Proc Natl Acad Sci U S A. 1978. PMID: 276864 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
