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. 1968 Jan 19;159(3812):314-6.
doi: 10.1126/science.159.3812.314.

Histone acetylation in insect chromosomes

V G Allfrey, B G Pogo, V C Littau, E L Gershey, A E Mirsky

Histone acetylation in insect chromosomes

V G Allfrey et al. Science. .

Abstract

Acetylation of histones takes place along the salivary gland chromosomes of Chironomus thummi when RNA synthesis is active. It can be observed but not measured quantitatively by autoradiography of chromosome squashes. The "fixatives" commonly used in preparing squashes of insect chromosomes preferentially extract the highly acetylated "arginine-rich" histone fractions; the use of such fixatives may explain the reported absence of histone acetylation in Drosophila melanogaster.

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