doi: 10.1084/jem.127.2.341.
Studies on the pathogenesis of fever. XVI. Purification and further chemical characterization of granulocytic pyrogen
- PMID: 5635382
- PMCID: PMC2138451
- DOI: 10.1084/jem.127.2.341
Item in Clipboard
Studies on the pathogenesis of fever. XVI. Purification and further chemical characterization of granulocytic pyrogen
J Exp Med.
.
Abstract
Small quantities of highly purified granulocytic pyrogen have been separated from contaminating proteins by disc electrophoresis in polyacrylamide gel. The biologically active material thus isolated was shown to be electrophoretically homogeneous at pH 9 and pH 3.8. Earlier work on the chemical properties of the pyrogen molecule has been extended to include: (a) estimation of its molecular weight by gel filtration; (b) demonstration of free sulfhydryl groups essential for its biological activity; and (c) evidence that it is not inactivated by exhaustive extraction with ethanolether or n-heptane.
Similar articles
-
Studies on the pathogenesis of fever. XVII. The cationic control of pyrogen release from exudate granulocytes in vitro.J Exp Med. 1970 Jan 1;131(1):165-78. doi: 10.1084/jem.131.1.165. J Exp Med. 1970. PMID: 5409945 Free PMC article.
-
Studies on the pathogenesis of fever. 18. Activation of leukocytes for pyrogen production.J Exp Med. 1970 Jan 1;131(1):179-88. doi: 10.1084/jem.131.1.179. J Exp Med. 1970. PMID: 4902892 Free PMC article.
-
Synthesis of endogenous pyrogen by rabbit leukocytes.J Exp Med. 1973 May 1;137(5):1263-74. doi: 10.1084/jem.137.5.1263. J Exp Med. 1973. PMID: 4573840 Free PMC article.
-
[Leukocyte pyrogens].Zh Mikrobiol Epidemiol Immunobiol. 1977 Nov;(11):50-3. Zh Mikrobiol Epidemiol Immunobiol. 1977. PMID: 339615 Review. Russian. No abstract available.
-
Pyrogen release in vitro by lymphoid tissues from patients with Hodgkin's disease.Yale J Biol Med. 1974;47(2):101-12. Yale J Biol Med. 1974. PMID: 4611062 Free PMC article. Review.
Cited by
-
Mechanism of fever induction in rabbits.Infect Immun. 1976 Nov;14(5):1130-7. doi: 10.1128/iai.14.5.1130-1137.1976. Infect Immun. 1976. PMID: 185148 Free PMC article.
-
Studies on the pathogenesis of fever. XX. Suppression and regeneration of pyrogen-producing capacity of exudate granulocytes.J Exp Med. 1970 Jul 1;132(1):127-33. doi: 10.1084/jem.132.1.127. J Exp Med. 1970. PMID: 5508243 Free PMC article.
-
Studies on the mechanism of endogenous pyrogen production. II. Role of cell products in the regulation of pyrogen release from blood leukocytes.Infect Immun. 1974 Sep;10(3):451-7. doi: 10.1128/iai.10.3.451-457.1974. Infect Immun. 1974. PMID: 4426696 Free PMC article.
-
Studies on the pathogenesis of fever. XVII. The cationic control of pyrogen release from exudate granulocytes in vitro.J Exp Med. 1970 Jan 1;131(1):165-78. doi: 10.1084/jem.131.1.165. J Exp Med. 1970. PMID: 5409945 Free PMC article.
-
Application of a TLR overexpression cell model in pyrogen detection.Biotechnol Bioeng. 2019 Jun;116(6):1269-1279. doi: 10.1002/bit.26936. Epub 2019 Feb 20. Biotechnol Bioeng. 2019. PMID: 30684361 Free PMC article.
