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. 1968 Jan;14(1):69-79.

Antibody production studied by means of the localized haemolysis in gel (LHG) assay. II. Assay procedure

H H Wortis, R B Taylor, D W Dresser

Antibody production studied by means of the localized haemolysis in gel (LHG) assay. II. Assay procedure

H H Wortis et al. Immunology. 1968 Jan.

Abstract

The localized haemolysis in gel (LHG or plaque) assay has been investigated and it has been shown that the best condition for the assay is a plate made up from a standard tissue culture medium (199) in an agarose gel. Several convenient but sub-optimal alternatives are described. Other variables that were investigated were the concentration of indicator erythrocytes in the assay plate, the amount of complement added, the incubation time and the neutralization of xenogeneic developing serum by free mouse serum γ-globulins. The reasons for using log(x+1) transformed data for statistical analysis are also presented.

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References

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